Hematogenesis Adaptation to Long-Term Hypoxia Acclimation in Zebrafish (Danio rerio)

When fish live in the wild or are cultured artificially, they will inevitably suffer from hypoxia. At same time, blood physiological indexes represent state of fish. In order to study effect long-term hypoxia acclimation on hematogenesis, we zebrafish embryos into adulthood a incubator (1.5 ± 0.2 mg/L). Then compared hematological parameters normoxia and conditions. Transcriptome sequencing analysis main hematopoietic tissue, head kidney, was also between two groups. Results showed that number erythrocytes increased significantly acclimated group, while size several cell types, such as red cells, eosinophils, basophils, small lymphocytes thrombocytes, decreased significantly. The transcriptomic comparisons revealed there were 6475 differentially expressed genes (DEGs) A Gene Ontology (GO) annotation Kyoto Encyclopedia Genes Genomes (KEGG) pathway hematopoiesis proliferation signaling most enriched pathways kidney zebrafish. addition, many involved process higher levels expression zebrafish, when considered together, these data allowed us conclude can promote which resulted production. Higher numbers cells allow for better adaptation hypoxic environment. conclusion, this provides basis in-depth understanding effects hematogenesis species.